Effects of Chiral Gold Nanoflowers on Polymerase Chain Reaction

In this paper, we reported that chiral gold nanoflowerswith the concentration of 0.08mMcan enhance the specificity of polymerase chain reaction (PCR) and eliminate unspecific products, and the efficiency of PCR declinesin some extentand the melt temperature of PCR products also raises slightly, which may be associated with the enhancement of the combining power of primers and ssDNA templates and the inner stability of dsDNAby chiral gold nanoflowers.The phenomenahave great potential in applications such as ultrasensitive specific detection of DNA, and SNPs.