Extracellular Synthesis of Silver Nanoparticles by Endophytic Bordetella SP. Isolated from Piper Nigrum and Its Antibacterial Activity Analysis

Roshmi Thomas, B Jasim, Jyothis Mathew, EK Radhakrishnan

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Abstract

In the current study, various endophytic bacterial isolates from Piper nigrum were explored for its nanoparticle synthesizing property. Very interestingly, one of the isolate which is a strain of Bordetella sp. was found to have the ability to form silver nanoparticles at room temperature within 24 hours by extracellular method. This was confirmed by the presence of a peak at 460 nm by UV- Vis absorption analysis and such a result is the indication of surface  plasmon resonance of silver nanoparticles. Further characterization of nanoparticles was carried out by Scanning electron microscopy (SEM) analysis. This confirmed the presence of silver nanoparticles with size less than 100nm. The antibacterial potential of silver nanoparticles synthesized was also tested against pathogens like Salmonella paratyphi, Vibrio cholerae and Staphylococcus aureus. So the current study is the demonstration of an efficient synthesis of silver nanoparticles by Bordetella sp. and their potential as nanomedicine to manage pathogenic bacteria.

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Nano Biomedicine and Engineering.

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