Evaluation of Antibacterial Activity and Cytotoxicity of Green Synthesized Silver Nanoparticles Using Scoparia Dulcis

Green synthesis of silver nanoparticles (AgNPs) is gaining momentum in the field of nano-research.Scoparia dulcis leaves were used as a reducing agent for the synthesis of silver nanoparticles froman aqueous solution of silver nitrate (AgNO3). Synthesized AgNPs were characterized by UV-Visspectroscopy, XRD, SEM with EDAX and TEM. UV-VIS surface plasmon resonance spectroscopywas observed at 430 nm. XRD data depicts that the NPs are crystalline in nature. The EDAX dataindicate that 63.76% presence of Ag metal. The TEM & SEM results indicate that size of the AgNPsranges from 15-25 nm. The results also support that spherical shape of the nanoparticles. In addition,the NPs are in polydispersed condition. The antimicrobial activities indicate significant inhibitionof the growth of three pathogenic bacteria such as Pseudomonas ariginosa, Bacillua subtillis andStaphylococcus aureous. Cytotoxicity of this nanoparticle showed that this Ag-NP also has morecytotoxic effect on a lung cancer cell line, A549 cells compared to ovarian cancer cell line, PA1indicating a possible therapeutic use of this AgNP.