How to Resolve Nucleic Acids with High Separation Performance by Pulsed Filed Capillary Electrophoresis

Zhenqing Li, Dawei Zhang *

 

Engineering Research Center of Optical Instrument and System, Ministry of Education and Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology.

 

* Corresponding author. E-mail: dwzhang@usst.edu.cn

 

Presented: 1st International Symposium of Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument (SHIIRC). Shanghai, China, Aug. 7, 2017; Published: Nov. 17, 2017

 

Citation: Zhenqing Li, Dawei Zhang, How to Resolve Nucleic Acids with High Separation Performance by Pulsed Filed Capillary Electrophoresis. Nano Biomed. Eng., 2017, Special Issue: 284.

 

Abstract

Nucleic acid separation is an important technology in molecular biology, following by polymerase chain reaction (PCR) analysis or before cloning, sequencing, northern or southern blotting. So far, high performance liquid chromatography (HPLC), capillary electrophoresis (CE), and slab gel electrophoresis (SGB) are three common methods used for the separation of DNA. In HPLC, the separation mechanism involves partitioning of analytes between a mobile and stationary phase. Besides the separation performance, CE are deemed to have distinct advantages over HPLC, because instrumentation of HPLC is rather complex compared to the simplicity of CE or SGE system. Furthermore, the elution of large molecules can only be achieved by changing the selectivity during separation process (e.g. gradient elution), and expensive prepacked columns are required which have limited life time in HPLC. However, the short and long nucleic acids (DNA or RNA) cannot always be resolved with high separation performance simultaneously. In this report, we demonstrated our research on how to resolve nucleic acids with high separation performance by pulsed field capillary electrophoresis.

 

Copyright© Zhenqing Li, Dawei Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

PDF