Comparison of Ultracentrifugation and Prekit-Exo Kit for the Identification of Exosomes Extracted from Serum of Gastric Cancer Patients

Xiaoqing Qian 1, Feng Xie 2, Wenxiu Hou 1, Tianliang Li 1, Fangfang Xia 1, Daxiang Cui 1, 3*

 

1 Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, School of Electronic Information and Electronical Engineering, 800 Dongchuan Road, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

2 Department of Cardiothoracic Surgery, Changhai Hospital,Shanghai 200240, P. R. China.

3 National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University, 800 Dongchuan RD, Shanghai 200240, P. R. China.

 

* Corresponding author. E-mail: dxcui@sjtu.edu.cn

 

Presented: 2018 Chinese Conference on Oncology. Shenyang, China, Aug. 17-19, 2018; Published: Oct. 18, 2018

 

Citation: Xiaoqing Qian, Feng Xie, Wenxiu Hou, Tianliang Li, Fangfang Xia, and Daxiang Cui, Comparison of Ultracentrifugation and Prekit-Exo Kit for the Identification of Exosomes Extracted from Serum of Gastric Cancer Patients. Nano Biomed. Eng., 2018, Special Issue: 318.

 

Abstract

Objective: To compare ultracentrifugation and Prekit-exo Kit in identifying the characteristics of exosomes extracted from Serum of breast cancer patients.

 

Methods: Serum samples were collected and extracted by ultra-high speed centrifugation (Ultra-exo) and Precipitation Kit (Prekit-exo) respectively. The biological morphology of exosome was observed by negative coloring matter with transmission electron microscopy, and the particle size and the distribution were detected by nano particle size tracking instrument (NTA). Results Bio-transmission electron microscopy, revealed that the morphologys in the ultracentrifugation-extracted exosomes were better than those in the Prekit-exo-extracted exosomes. Results showed that the number of the ultracentrifugation-extracted exosomes was more than that from the Prekit-exo-extracted exosomes.Also NTA(Nanoparticle Tracking Analysis)shows the number of ultracentrifugation-extracted exosomes is more than  number of  Prekit-exo-extracted exosomes. Western Blot results show that protein of exosomes obtained by Prekit-exo method are more and miscellaneous than Ultra-exo method. 

 

Conclusion: Exosomes from Serum of breast cancer patients isolated by ultracentrifugation and precipitation are different in morphologys and numbers, and ultracentrifugation method is superior to precipitation method.

 

Keywords: Exosome; Ultracentrifugation; Kit; Precipitation 

 

Copyright© Xiaoqing Qian, Feng Xie, Wenxiu Hou, Tianliang Li, Fangfang Xia, and Daxiang Cui. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

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