Resumption of Meiotic Maturation of Oocytes, Pre- and Post-Implantational Embryonic Mortality under Conditions of Experimental Glomerulonephritis and Treatment of Silver Nanoparticles

Taras Viroslavovich Blashkiv, Maria Sergeeevna Stupchuk, Valentine Aleksandrovna Sribna, Oksana Mykolajivna Kaleynykova, Nataliya Georgejivna Grushka, Tetyana Yurievna Voznesenska

Abstract

The aim of the current study was to estimate the resumption of meiotic maturation of oocytes, pre- and post-implantation mortality of embryos under conditions of experimental glomerulonephritis and intravenous treatment of silver nanoparticles in mice. Experimental glomerulonephritis in mice was achieved by immunization of white laboratory mice of the first generation with a kidney antigen suspension derived from a parent. The treatment was carried out in the following way: Kidney antigen suspension - intraperitoneal three times 1 time per day; the procedure was repeated in 3 weeks, one time intraperitoneally with the same dose (10 mkL of suspension per 10 grams of body weight of the animal). Silver nanoparticles (AgNPs, 30 nm) - intravenous treatment three times: 1 time per day for 1 h before immunization of animals with suspension of kidney antigen; as well as in 3 weeks once with the same dose (2 mg/kg).Thus, the number of oocytes that resumed meiosis in vitro from animals under experimental glomerulonephritis decreased as compared to the numbers in control animals; no inhibition of meiotic resumption of oocytes of animals was established under conditions of AgNPs treatment; the AgNPs treatment under conditions of experimental glomerulonephritis increased the number of oocytes that resumed meiosis in vitro compared with such values in the control and under conditions of experimental glomerulonephritis. The value of post-implantation embryonic mortality increased under conditions of experimental glomerulonephritis; no infertility of implantation in females of mice under conditions of AgNPs treatment; the AgNPs treatment under conditions of experimental glomerulonephritis decreased post-implantation mortality of embryos.

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