Anti-Proliferative Activity and Tubulin Targeting of Novel Micro and Nanoparticles Complexes of 4-Amino-3-Thion-1,2,4-Triazole Derivatives

Atheer Ali, Rehab Al-Hassani, Dhia Hussain, Majid Jabir, Hamid Meteab

Abstract

In this study, novel micro and nanoparticle complexes of Ag(I), Ni(II), and Pd(II) ion with new asymmetrical Schiff base triazole ligand (4-(((3-mercapto-5-(naphthalen-1-ylmethyl)-4H-1,2,4-triazol-4-yl)imino)methyl)phenol) were prepared. The Schiff base micro complexes were identified using Fourier-transform infrared spectroscopy (FTIR), Ultraviolet–visible spectroscopy (UV-Vis), flame atomic absorption, elemental analysis C.H.N.S, conductivity measurements and magnetic susceptibility. New nanoparticles Schiff base triazole ligand (4-(((3-mercapto-5-(naphthalen-1-ylmethyl)-4H-1,2,4-triazol-4-yl)imino)methyl)phenol) ligand and Ag(I), Ni(II) and Pd(II) complexes were synthesized as a novel compounds by using sonication method, and were fully characterized by using FTIR, atomic force microscopy (AFM), scanning electron microscope (SEM), and X-ray powder diffraction (XRD). The antioxidant activity of tested compounds was tested using DPPH assay. The effect of synthetic novel compounds on cancer cell line MCF-7 proliferation was measured by MTT assay. The ability of synthetic novel compounds in induction of apotosis was achieved using acridine orange/ethidium bromide (AO/EB) stains. We found that the synthetic novel compounds had the potential to inhibit growth of cancer cell at low concentrations. The effect of synthetic compounds on cell growth and proliferation of MCF-7 cells was associated with cell cycle arrest, and increased apoptosis. Our results showed that the synthetic novel compounds inhibited cancer cell line proliferation with a mechanism of action similar to that of other tubulin inhibitors. In conclusion, the results of this study indicate that synthetic novel compounds represent a new chemo type with a novel mechanisms of action and that it has the potential to be developed for tumor therapy.

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Nano Biomedicine and Engineering.

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