Histological, Biochemical and DNA Changes in the Liver of Male Albino Rats Treated with Silver Nanoparticles

Abed Baraaj

Abstract

Sliver nanoparticles (Ag NPs) are produced industrially and commercially available in recent time. Silver and its nanoparticles are widely applied to consuming medical purposes. Silver nanoparticles are able to resemble cellular components and typical  proteins, which helps the nanoparticles to cross natural barriers in human body. For this reason, the current study was designed to investigate the damage caused by silver nanoparticles, especially in liver which is one of the most targeted organs of nanoparticles as a result of their accumulation and damages caused in various aspects such as tissue, functional ability and DNA of liver cells. 30 albino male rats were used for this study and were divided into 3 main groups. The first group represented control and a normal saline solution was given, while the second group was given silver nanoparticles at a concentration of 2 mg/kg of body weight, and the third group was given silver nanoparticles at a concentration of 3 mg/kg of  body weight. Each concentration of Ag NPs was dissolved in 3 mL of the normal saline and given directly to the experimental animal’s stomach by feeding tube with diameter of 0.6 cm and every day for 10 weeks. The results in the current study showed histopathological change in liver tissue such as large granulomatous lesion with an increase in macrophage numbers, as also shown by necrotic hepatocytes with dilation of sinusoids. The deformation of architecture in the parenchyma appeared in both concentrations. The biochemical parameters investigation in the liver clarified increased level of enzymes accordance with doses of Ag NPs. The damage was identified in DNA of hepatocytes by comet assay and the degree of damage was evident through increasing the numbers of tailed nuclei and in strand breaks of DNA. The reactive oxygen species (ROS) generation had a key role in the events of structural and functional disorders in the liver of experimental animals.

Nano Biomedicine and Engineering.

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