Single Cell Manipulation Technology

With microfluidic technique emerging, cell manipulation technology combines with microfluidicbecome a promising tool for single-cell-level manipulation. To obtain a kind of or single pure targetcell for eliminating interference of useless cells in the trial of molecular biology, genetic analysis,proteomics and single cell analysis, various cell manipulation techniques have been developedfor recovery specific cells. In this review, we introduce the principles of each cell manipulationtechnology and overlook the latest achievements of cell manipulation technique by categorizingexternally applied manipulation forces: optical, electrical, magnetic, acoustic, mechanical. We alsosummarize the advantages and drawbacks of each cell manipulation technique.